DOS 2018

DOS Kongressen 2018 · 259 Expression of Collagen mRNA isoforms in Primary Frozen Shoulder Line Marker, Mads Okholm, Peter Schjerling, Michael Krogsgaard Institute of Sports Medicine, Bispebjerg Hospital; Intstitue of Sports Traumatology, Bispebjerg Hospital Background: Primary frozen shoulder is a painful condition with a decreased range of motion making the disease debilitating. The condition can be divided in to three phases that differs in clinical presentation. Macroscopically changes have shown contracture and fibrosis in the shoulder. Type I and III collagen has been the main investigating target so fare, and both have seen to be increased in the capsule and the surrounding ligaments. Purpose / Aim of Study: The aim of this study was to investigate the expres- sion of different types of collagens in patients with primary frozen shoulder, and to see if there was any difference in the expression of collagen between the three phases of idiopathic frozen shoulder. Materials and Methods: This study used a case-control study design, ana- lyzing patients undergoing arthroscopic treatment of iprimary FS and control patients being treated for subacromial impingement. Biopsies were taken from the medial glenohumeral ligament and the anterior capsule from all subjects. mRNA levels were analyzed using real-time PCR. Nonparametric test were used for the statistics. Findings / Results: The results from this current study show that many genes for different types of collagens, e.g. type 1, 3, 4, 5, 6, 14, are activated in pri- mary FS in the anterior capsule and the medial glonohumeral ligament. We also saw that there are no significant differences in the expression of collagen mRNA between the three phases. Conclusions: Our findings suggest that there is a general increased synthesis of collagens in all phases of primary frozen shoulder, and therefore that the progression in the clinical presentation of frozen shoulder is not due to changes in collagen production. 200.