DOS 2018

DOS Kongressen 2018 · 237 The osteogenic differentiation potential of mesenchymal stem cells isolated from both knees of patients with unilateral trauma or osteoarthritis of the knee Casper Falster, Simon Poulsen, Arne Storås, Henrik Schroeder, Jesper Vinther, Moustapha Kassem, Uffe Jørgensen Stem cell research unit (KMEB), Department of Endocrinology, Odense University Hospital; Department of Orthopedic Surgery and Sports traumatol- ogy, Odense University Hospital; Department of Orthopedic Surgery, Næstved Hospital Background: Bone fractures are a common cause of hospital admission for pa- tients of all age groups. In recent years, regenerative medicine has introduced a possible new addition to already established treatments. Evidence suggests that autologous mesenchymal stem cells (MSC) can enhance bone regeneration, by differentiating into bone-forming osteoblasts. Purpose / Aim of Study: This study aims to investigate if MSCs isolated from bone marrow in sites of recent trauma or osteoarthritis (OA), exhibit reduced proliferative capacity and osteogenic differentiation capability in- vitro, com- pared to MSCs isolated from non- traumatic and non-OA sites. If these are of reduced quality, clinicians should prioritize bone marrow from unafflicted sites. Materials and Methods: 17 patients were enrolled. 7 had recent unilateral trauma to the knee, requiring arthroscopy. 10 had x- ray verified unilateral OA and were scheduled for total knee arthroplasty. MSCs were isolated from bone marrow aspirated from both distal femurs of all participants. In-vitro osteo- genic activity was assessed through alkaline phosphatase measurement, RNA- expression and alizarin red staining. Proliferation was measured using a growth curve. Findings / Results: 29 out of 34 primary cultures were succesful. There was no difference in mononuclear cell yield of aspirates or yield of MSCs from pri- mary culture between non-OA and OA knees or non-traumatic and traumatic knees. There was no significant difference in in-vitro osteogenic capability or proliferative capacity. Conclusions: We found no difference in proliferation or osteogenic differentia- tion of MSCs isolated from both distal femurs of patients with unilateral trauma or OA of the knee, suggesting that MSCs isolated from sites afflicted by OA or trauma, can be utilized for bone regeneration with identical results as MSCs isolated from uninjured sites. 178.