132
· DOS Abstracts
Vancomycin is superior to Plectasin against
Staphyloccus aureus periprosthetic osteomyelitis
in rats.
Niels H. Søe, Nina Vendel-Jensen, Asger Lundorff-Jensen, Janne Koch,
Steen Sejer Poulsen, Helle Krogh Johansen
Hand Section,Department of Orthopaedic, Gentofte University Hospital;
Department of Anaesthesiology,Intensive care and Operations, Gentofte
University Hospital; Faculty of Health and Medical Sciences, University
of Copenhagen; Biomedical Department,Panum, Institute, University of
Copenhagen; Department of Clinical Microbiology, Rigshospitalet, Denmark
Background:
Commonly used antibiotics cannot always control S. aureus as-
sociated infections in orthopaedic implants
Purpose / Aim of Study:
We investigated the ability of Vancomycin and
Plectasin to eradicate S. aureus in a knee prosthesis model of osteomyelitis in
rats. We compared Plectasin in a 20 and 40 mg concentration to Vancomycin
40 mg, all antibiotics were given i.p. daily. Plectasin is a peptide antibiotic with
therapeutic potential from a saprophytic fungus.
Materials and Methods:
Thirty Sprague-Dawley rats had prosthesis inserted
and divided into three groups (N=10) Vancomycin including 2 controls and two
Plectasin groups (N= 10) 20 mg and (N= 10) 40 mg including 2 controls in each
groups. All groups were infected with S. aureus MN8,ica+103 in the tibia and the
femur marrow before insertion of the prosthesis. Control rats were given NaCl
i.p. After two weeks, the rats were sacrificed and all specimens were analysed.
Findings / Results:
One rat in the 20 mg Plectasin group and four in the 40
mg Plectasin group died of anaphylactic shock (histamine release). The 20 and
40 mg Plectasin groups both showed a decrease of bacteria but it was not as
efficiently eradicated as in the Vancomycin group.
Conclusions:
Plectasin treatment against S. aureus osteomyelitis reduced the
infection. However, Plectasin released histamine strongly after one day and
some of the rats died especially in the 40 mg group. In contrast, Vancomycin
reduced the infection significantly in almost of the parameters.
79.
